Calcium Ion Dynamics after Dexamethasone Treatment in Organotypic Cultured Hippocampal Slice

• 영문명: Calcium Ion Dynamics after Dexamethasone Treatment in Organotypic Cultured Hippocampal Slice
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Hee-Jung Chae Tong-Ho Kang Ji-Ho Park
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제9권 제6호, 363~370쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2005.01.01

영문 초록

It is imperative to analyse brain injuries directly in real time, so as to find effective therapeutic compounds to protect brain injuries by stress. We established a system which could elucidate the real time Ca^2⁢ dynamics in an organotypic cultured hippocampal slice by the insults of artificial stress hormone, dexamethasone. The real time Ca^2⁢ dynamics could continuously be detected in cornus ammonis 3 (CA3) of the organotypic hippocampus for 8 hours under confocal microscopy. When dexamethasone concentration was increased, the Ca^2⁢ was also increased in a dose dependent manner at 1∼100 μM concentrations. Moreover, when the organotypic cultured hippocampal slice was treated with a glutamate receptor antagonist together with dexamethasone, the real time Ca^2⁢ dynamics were decreased. Furthermore, we confirmed by PI uptake study that glutamate receptor antagonist reduced the hippocampal tissue damage caused by dexamethasone treatment. Therefore, our new calcium ion dynamics system in organotypic cultured hippocampal slice after dexamethasone treatment could provide real time analysis method for investigation of brain injuries by stress.

키워드

Calcium dynamics Hippocampus Stress Dexamethasone Glutamate antagonist