Gene Transfer of Cu/ZnSOD to Cerebral Vessels Prevents Subarachnoid Hemorrhage-induced Cerebral Vasospasm

• 영문명: Gene Transfer of Cu/ZnSOD to Cerebral Vessels Prevents Subarachnoid Hemorrhage-induced Cerebral Vasospasm
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Mi Ran Yun Dong Eun Kim Hye Jin Heo Ji Young Park Ji Young Lee Sun Sik Bae Chi Dae Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제9권 제6호, 327~332쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2005.01.01

영문 초록

The preventive effects of gene transfer of human copper/zinc superoxide dismutase (Cu/ZnSOD) on the development of cerebral vasospasm after subarachnoid hemorrhage (SAH) were examined using a rat model of SAH. An experimental SAH was produced by injecting autologous arterial blood twice into the cisterna magna, and the changes in the diameter of the middle cerebral artery (MCA) were measured. Rats subjected to SAH exhibited a decreased diameter with an increased wall thickness of MCA that were significantly ameliorated by pretreatment with diphenyleneiodonium (DPI, 10μM), an inhibitor of NAD(P)H oxidase. Furthermore, application of recombinant adenovirus (100μl of 1⁓10¹⁰ pfu/ml, intracisternally), which encodes human Cu/ZnSOD, 3 days before SAH prevented the development of SAH-induced vasospasm. Our findings demonstrate that SAH-induced cerebral vasospasm is closely related with NAD(P)H oxidase-derived reactive oxygen species, and these alterations can be prevented by the recombinant adenovirus-mediated transfer of human Cu/ZnSOD gene to the cerebral vasculature.

키워드

Cu/ZnSOD SAH NAD(P)H oxidase Cerebral vasospasm