PKA-Mediated Regulation of B/K Gene Transcription in PC12 Cells

• 영문명: PKA-Mediated Regulation of B/K Gene Transcription in PC12 Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Mi-Hyun Choi Ho-Shik Kim Sung-Ho Choi Mi-Young Kim Yoon-Seong Jang Young-Min Jang Jeong-Hwa Lee Seon
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제9권 제6호, 333~340쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2005.01.01

영문 초록

B/K protein is a novel protein containing double C2-like domains. We examined the specific signaling pathway that regulates the transcription of B/K in PC12 cells. When the cells were treated with forskolin (50μM), B/K mRNA and protein levels were time-dependently decreased, reaching the lowest level at 3 or 4 hr, and thereafter returning to the control level. Chemicals such as dibutyryl-cAMP, cell- permeable cyclic AMP (cAMP) analogue and CGS21680, adenosine receptor A_2A agonist, also repressed the B/K transcription. However, 1,9-dideoxyforskolin did not show inhibitory effect on B/K transcription, suggesting direct involvement of cAMP in the forskolin-induced inhibition of B/K transcription. Effect of forskolin, dibutyryl cAMP and CGS21680 was significantly reduced in PKA-deficient PC12 cell line (PC12-123.7). One cAMP-response element (CRE)-like sequence (B/K CLS) was found in the promoter region of B/K DNA, and electrophoretic mobility shift assay indicated its binding to CREM and CREB. Forskolin significantly suppressed the promoter activity in CHO-K1 cells transfected with the constructs containing B/K CLS, but not with the construct in which B/K CLS was mutated (AC：TG). Taken together, we suggest that the transcription of B/K gene in PC12 cells may be regulated by PKA-dependent mechanism.

키워드

B/K protein Cyclic AMP Protein kinase A PC12 cells Forskolin cAMP response element