타액분비 및 선혈류 조절에 대한 내인성 산화질소의 역할

• 영문명: Role of Endogenous Nitric Oxide in the Control of Salivary Secretion and Blood Flow
• 발행기관: 대한생리학회-대한약리학회
• 저자명: 김원재(Won jae Kim) 남상채(Sang Chae Nam) 김미원(Mi won Kim)
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제6호, 809~816쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

The present study was designed to investigate whether endogenous niπic oxide(EDNO) is involved in submandibular vasodilation and salivation induced by parasympathetic nerve stimulation. Effects of NW-nitro-Larginine-methyl ester (L-NAME) which blocks the synthesis of EDNO from L-arginine on the submandibular vasodilation and salivation induced by chorda stimu1ation or administration of various vasodilators were examined in anesthetized cats. Effect of L-NAME on K+ efflux induced by carbachol was also examined using the excised submandibular slice in vitro. In the submandibular slices， acetylcholine(lO 5 molfL) or vasoactive intestinal polypeptide(VIP， 10 5 molfL) increased N02 contents， which was prevented by pretreatment with L-NAME. Salivary secretion in response to the chorda stimulation(3 V， 1 msec， 1O�20 Hz) was completely blocked by treatment with atropine(l mgjkg). Increased blood flow response to the low frequency(l， 2， 5 Hz) stimulation was significant1y reduced， whereas the blood flow induced by the higher frequency(lO， 20 Hz) stimu1ation was not affected. Lingual-arterial infusion of L-NAME(lOO mgjkg) significant1y diminished the vasodilatory and salivary responses to the chorda stimulation at all stimuli frequencies used. Intra-arterial infusion of L-NAME(100 mgjkg markedly diminished the asodilatory responses to acetylcholine(5 llgjkg)， VIP(5 llgjkg) or br때ykinin(5 llgjkg). In the excised submandibular lice，K+ efflux in response to carbachol(1O-5 molfL) was significant1y decrease by pretreatment with L-NAME(1O-5molfL). In the is이ated submandibular artery precontracted with phenyleplrrine(1O-5 molJL) ， the asore1axation induced by ACh(lO 7 molfL) was reversed into a contraction by methylene blue(lO 4 molfL). These resu1ts suggest that EDNO may play an important role in vasodilation and secretion of the submandibular gland.

키워드

Endogenous nitric oxide Salivary gland L-NAME B100d flow Salivation