사람 Neuroblastoma SH-SY5Y 세포주에서 Opiate 내성에 의한 c-myc 유전자 표현

• 영문명: The c-myc Expression on the Opioid Tolerance in Human Neuroblastoma SH-SY5Y Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: 김수경(Soo kyung Kim) 박창교(Chang kyo Park) 권지윤(Gee youn Kwon) 서성일(Sung il Suh)
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제6호, 691~697쪽, 전체 7쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

The mechanisms underlying opiate tolerance and dependence are not fully understood. We used human neuroblastoma SH-SY5Y cells as a model system for studying effects of morphine tolerance and withdrawal on c-myc induction and cAMP levels. It has been reported that regulation of c-fos by acute and chronic morphine withdrawal is mediated through alterations in CREB transcription factor.

In this study, we examined the effects of morphine tolerance on c-myc expression and cAMP concentrations. The activation of opiate receptors by an acute morphine administration resulted in an increase in c-myc mRNA and a decrease in cAMP concentrations in a dose-dependent manner (5, 10, 15, and 20 ㄍM). On the other hand, the chronic treatment of morphine (10 ㄍM for six days) did not induce the elevated expression of c-myc mRNA. The c-myc expression was slightly inhibited in comparison with that of the acute morphine response. However, cAMP concentrations were increased with regard to morphine withdrawal response.

These results suggest that the alterations in c-myc expression might imply a significant opiate regulation relating to morphine tolerance. This observation differs from increased expression of c-fos via regulation of cAMP pathway.

키워드

Human neuroblastoma SH-SY5Y cells c-myc cAMP