고양이의 신장에서 Ethacrynic Acid가 PAH 분비에 미치는 영향

• 영문명: Effect of Ethacrynic Acid on Renal Tubular Secretion of PAH in Anesthetized Cat
• 발행기관: 대한생리학회
• 저자명: 김용근(Kim, Y.K.) 정진섭(Jung, J.S.) 김주헌(Kim, J.H.) 서덕준(Suh, D.J.) 이상호(Lee, S.H.)
• 간행물 정보: 『대한생리학회지』제16권 제2호, 177~186쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1982.12.31

영문 초록

The effect of ethacrynic acid (EA) on the renal secretion of PAH was examined in cat kidney. C_PAH and T_PAH were measured before and after infusion of EA (0.5 ~ 50mg/kg) through the femoral vein. The following results were obtained: 1) In the dosage range of 0.5 to 25 mg/kg, EA increased the urine flow, and sodium and potassium excretion in dose-dependent manner, but the glomelular filtration rate was decreased as the dosage of EA was increased. 2) C_PAH and T_PAH were decreased by EA in the dosage range of 3 to 25 mg/kg and 1 to 50 mg/kg, respectively, in dose¡¤dependent manner with the dosage to cause 50% inhibition of about 5 mg/kg. 3) With dosage of 0.5mg/kg, EA appeared to exert a great effect on diuretic response without the influence on T_PAH. At 10min after infusion of EA, a potent diuretic effect appeared, while T_PAH did not show a significant change. These results suggest that the action mechanism of EA on tubular secretion of PAH may be different from that on natriuresis. 4) With dosage of 5 mg/kg, EA did not inhibit the Na-K-ATPase activity in microsomal fractions from both cortex and medulla. 5) The double reciprocal plot (l/T_PAH versus l/P_PAH) suggested that EA inhibited the P AH secretion by a competitive pattern. However, probenecid, a prototypic inhibitor of the organic acid pump, had no influence on both the inhibitory effect of T_PAH and the natriuretic effect by EA. These results suggest that in vivo EA altered tubular secretion of P AH through interactions with receptors that are not identical with the Na-K-ATPase.