Vanadate가 골격근 sarcoplasmic reticulum의 Ca++-ATPase 및 Ca++ uptake에 미치는 영향

• 영문명: Effects of Vanadate on Activity of Ca⁺⁺Activated ATPase and Ca⁺⁺ Uptake of Sarcoplasmic Reticulum in Rat Skeletal Muscle
• 발행기관: 대한생리학회
• 저자명: 주순재(Joo, Soon-Jae) 한경희(Han, Kyung-Hee) 임중우(Leem, Joong-Woo) 강두희(Kang, Doo-Hee)
• 간행물 정보: 『대한생리학회지』제20권 제2호, 157~164쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1986.12.31

영문 초록

Since it has been reported that vanadate inhibits Ca⁺⁺-ATPase activity without affecting Ca⁺⁺ uptake, this study was undertaken to investigate the effects of vanadate on Ca⁺⁺-ATPase activity and Ca⁺⁺ uptake in the sarcoplasmic reticulum of rat skeletal muscle. The following results were obtained. 1) Ca⁺⁺ activated ATPase activity of the intact sarcoplasmic reticulum was significantly inhibited when vanadate was added to the incubation medium at concentration greater than 10^-6 M. However Mg⁺⁺-ATPase activity of the intact SR was not affected by vanadate at concentrations ranging from 10^-7 to 10^-4 M. Similarly, Ca⁺⁺-ATPase activity in sonicated sarcoplasmic reticulum was significantly reduced by vanadate at a concentration 10^-7 M or higher. 2) The uptake of Ca⁺⁺ by isolated sarcoplasmic reticulum was also inhibited by vanadate under the conditions where the turnover rate of Ca⁺⁺-ATPase was made to increase. These results suggest that the inhibition of Ca⁺⁺ uptake by vanadate may be correlated with that of Ca⁺⁺-ATPase if experimental conditions are properly set.