tyroFluoxetine Increases the Expression of NCAM140 and pCREB in Rat C6 Glioma Cells

• 발행기관: 대한신경정신의학회
• 저자명: MiRan Choi DongHoon Oh SeokHyeon Kim KyoungHwa Jung Nando Dulal Das YoungGyu Chai
• 간행물 정보: 『Psychiatry Investigation』제9권 제2호, 180~186쪽, 전체 7쪽
• 주제분류: 의약학 > 정신과학
• 파일형태: PDF
• 발행일자: 2012.06.30

영문 초록

Objective-Dysfunction of neural plasticity in the brain is known to alter neural networks, resulting in depression. To understand how fluoxetine regulates molecules involved in neural plasticity, the expression levels of NCAM, NCAM140, CREB and pCREB, in rat C6 glioma cells after fluoxetine treatment were examined. Methods-C6 cells were cultured after 20 min or after 6, 24 or 72 h treatments with 10 μM fluoxetine. Immunocytochemistry was used to determine the effect of fluoxetine on the expression of NCAM. Western blot analysis was used to measure the expression levels of NCAM140 and CREB and the induction of pCREB after fluoxetine treatment. Results-NCAM expression following 72-h fluoxetine treatment was significantly increased around cell membranes compared to control cells. Cells treated with fluoxetine for 6 and 72 h showed a significant increase in NCAM140 expression compared to cells treated for 20 min. The level of pCREB in the cells treated with fluoxetine for 72 h not only increased more than 60%, but was also significantly different when compared with the other treatment times. The 72-h fluoxetine treatment led to the increase of NCAM140 and the phosphorylation of CREB in C6 cells. Conclusion-Our findings indicate that fluoxetine treatment regulates neuronal plasticity and neurite outgrowth by phosphorylating and activating CREB via the NCAM140 homophilic interaction-induced activation of the Ras-MAPK pathway.

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키워드

Antidepressant C6 cells Fluoxetine NCAM140 pCREB