백서 뇌에서 전기경련충격에 의한 AP-1 결합 활성도의 변화

• 영문명: Changes of AP-1 Binding Activities in Rat Brain by Electroconvulsive Shock
• 발행기관: 대한신경정신의학회
• 저자명: 주연호 안용민 강웅구 구영진 김용식
• 간행물 정보: 『신경정신의학』제42권 제5호, 544~552쪽, 전체 9쪽
• 주제분류: 의약학 > 정신과학
• 파일형태: PDF
• 발행일자: 2003.09.30

영문 초록

Objectives：ECS could have therapeutic effects on psychiatric illnesses by inducing IEGs, which in turn regulates expression of their target genes. We observed AP-1 binding activity and identified AP-1 binding proteins in NMDAR1, late response gene of IEGs, which considered as the candidate gene for schizophrenia. Methods：By gel shift assay and supershift assay, we observed binding activities and AP-1 binding proteins in NMDAR1. Because IEGs are induced rapidly but transiently by external stimuli, there is a possibility that the expression of IEGs is negatively feedbacked by their own products via their AP-1 binding sites. For that purpose, we also observed AP-1 binding activity of c-fos and c-jun via gel shift and supershift assay. Results：ECS increased AP-1 binding activities of NMDAR1 gene, contributed by c-Fos and its related proteins. Peak of the increased binding was 60 minutes in both hippocampus and cerebellum. Though expression of c-Fos and c-Jun were increased by ECS, there were no changes in AP-1 binding activities after ECS. AP-1 sites of IEGs were binded by CREB, regardless of ECS. Conclusion：There is a possibility that ECS induced IEG expression, and then incresed expression of NMDR1 by binding of expressed IEGs to the AP-1 site of NMDAR1. ECS did not increase AP-1 binding activities of IEGs. This suggests that the regulation of IEGs’ expression can not be influenced mainly by AP-1 site.

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키워드

ECS (electroconvulsive shock) IEG (immediate early gene) AP-1·NMDAR1 (N-methyl-D-aspartate receptor)