Development of a stabilization buffer to enhance the physical stability of foot-and-mouth disease virus antigens

• 발행기관: 한국예방수의학회
• 저자명: Sungho Shin Yong Bin Kim Seung Heon Lee Hye Jun Jo Seon Woo Kim Jong-Hyeon Park Jaejo Kim
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.48, No.4, 219~223쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2024.12.31

국문 초록

Because intact FMDV particles (146S) are often unstable in vitro, stabilizing foot-and-mouth disease virus (FMDV) antigens remains a key challenge in studying viral charateristics. Therefore, finding optimal condition to stabilize the FMDV is essential. In this study, we investigated formulations and potentials of several stabilizers such as appropriate buffer, excipients, and storage conditions to enhance the stability of 146S. Inactivated FMDV O-Jincheon (O-JC) was dissolved in various buffer formulations, and stored at 4℃ for two months to evaluate quantity of 146S at every 2-week interval. Among phosphate buffered saline (PBS), Tris buffered saline (TBS), HEPES buffered saline (HBS), and MOPS buffered saline (MBS), PBS showed more effective 146S stabilization that showed 1.3-1.6 fold higher 146S fraction than TBS, HBS, and MBS after storage for 2 weeks. However, constant dissociations of 146S were observed in all formulations at 8 weeks. Compared with other FMDVs, A22 Iraq and SAT-1, in PBS, O-JC proved to be the least stable in PBS. A variety of excipients including carbohydrate, sugar alcohol, cryo-protectant were tested for the capability in protecting O-JC from dissociation. By adding 4-8% sucrose, more than 60% of 146S fractions were maintained at 8 weeks, those were at least 1.8 fold higher than the PBS-only control. Addition of 1% β-cyclodextrin showed synergistic enhancement in O-JC stability. As the results of this study, it could be suggested that the PBS-based buffer together with 4-8% sucrose + 2% sorbitol or 2% sucrose + 2% sorbitol + 1% β-cyclodextrin could help the better stability of the O-JC in vaccine preparation.

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키워드

Food-and-mouth disease virus 146S particles Stabilization Buffer formulation

참고문헌

• Prev Vet Med
• Cryobiology
• Arch Virol
• Protein Cell
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• Vaccine
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• Vaccines (Basel)
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• Nature
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• Vaccines (Basel)
• Curr Top Microbiol Immunol
• Vet Microbiol
• Sci Rep
• J Biol Chem
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• PLoS One
• Nat Commun
• Front Microbiol
• Vaccine