Developing DNA Markers based on Male-Specific Chromosomal Regions for Selecting Male Plants in Hop (Humulus lupulus)

• 영문명: Developing DNA Markers based on Male-Specific Chromosomal Regions for Selecting Male Plants in Hop (Humulus lupulus)
• 발행기관: 한국육종학회
• 저자명: Tae hyun Ha Jae Il Lyu So Young Yi Si-Yong Kang
• 간행물 정보: 『Plant breeding and biotechnology』Vol.12, 10~16쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2024.03.30

영문 초록

Hops are dioecious, with only female plants cultivated for resin production and primarily used in brewing. Therefore, early sex identification at the seedling stage is crucial for breeding and cultivation. Molecular marker-assisted selection facilitates rapid and reliable sex identification of hops. We developed molecular male markers by leveraging sequence information from male-specific regions in a designated public database. To assess the accuracy of sex identification using the newly generated markers, we performed PCR analysis on four cultivars of hops with known sexes. Seventy-eight percent of the tested PCR primers correlated with the male sex phenotype. Following optimization, four primer pairs were successfully converted into male-specific PCR markers.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgments

References

키워드

Hop Marker-assisted selection (MAS) Dioecious plant Sex identification Male-specific chromosomal region