Extraction of Lactate Dehydrogenase from Rice (Oryza sativa) and Corn (Zea Mays) for Alternative Lactic Acid Production Route

• 영문명: Extraction of Lactate Dehydrogenase from Rice (Oryza sativa) and Corn (Zea Mays) for Alternative Lactic Acid Production Route
• 발행기관: 한국육종학회
• 저자명: Astri Nur Istyami Putri Anggreyani Muhammad Alif Akbar Syach Arfanza Satria Joshua Michael Servilo Tarigan Tirto Prakoso
• 간행물 정보: 『Plant breeding and biotechnology』Vol.12, 1~9쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2024.03.30

영문 초록

Lactate dehydrogenase (LDH) is an enzyme found in nearly all living cells. It converts pyruvic acid to lactic acid as a subsequent step of glycerol metabolic pathway, thus LDH contributes to lactic acid biosynthesis from glycerol. LDH was reportedly active in rice (Oryza sativa) and corn (Zea mays) extract. This research aims to investigate the performance of LDH from rice extract and corn extract in pyruvic acid reduction to lactic acid. The effect of extraction temperature and extract concentration to lactic acid productivity was determined. Quantitative analysis of reaction product shows the presence of other products, which was predicted to be product of pyruvic acid oxidation and acetaldehyde. Activity of lactate dehydrogenase in pyruvic acid reduction was confirmed, suggesting a potential of further applications in lactic acid production.

목차

Introduction

Materials and Methods

Results

Discussion

Conclusion

Acknowledgments

References

키워드

Lactate dehydrogenase Lactic acid Pyruvic acid Oryza sativa Zea mays