학술논문
Combination of recombinant Brucella abortus dapB and gpm proteins induces host immunity against B. abortus 544 infection using a mouse model
이용수 5
- 영문명
- Combination of recombinant Brucella abortus dapB and gpm proteins induces host immunity against B. abortus 544 infection using a mouse model
- 발행기관
- 한국예방수의학회
- 저자명
- Tran Xuan Ngoc Huy Alisha Wehdnesday Bernardo Reyes Ched Nicole Turbela Aguilar Trang Thi Nguyen Said Abdi Salad Hu Jang Lee Jin Hur Dong-Kwan Lee Suk Kim
- 간행물 정보
- 『Journal of Preventive Veterinary Medicine』Vol.47, No.4, 171~178쪽, 전체 8쪽
- 주제분류
- 의약학 > 기타의약학
- 파일형태
- 발행일자
- 2023.12.31
4,000원
구매일시로부터 72시간 이내에 다운로드 가능합니다.
이 학술논문 정보는 (주)교보문고와 각 발행기관 사이에 저작물 이용 계약이 체결된 것으로, 교보문고를 통해 제공되고 있습니다.
국문 초록
영문 초록
The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
해당간행물 수록 논문
- Journal of Preventive Veterinary Medicine Vol.47, No.4 목차
- Staphylectomy using CO₂ laser for the treatment of nasopharyngeal stenosis in a cat
- Establishment of withdrawal time and analysis of didecyldimethylammonium chloride (DDAC) residue in edible tissues of broiler chickens by spraying DDAC disinfectant
- Non-surgical treatment of the cervical intervertebral disc disease in a dog with Chiari-like malformation
- Surgical resection of an olfactory groove meningioma using a 3D-printing technique in a dog
- Problems and proposed solution for the regulation of veterinary narcostic pharmaceuticals
- Evolutionary dynamic of HA gene of swine H1N2 influenza virus in Korea from 2009 to 2022
- Optimization of novel type of trivalent inactivated Salmonella bacterial vaccine prevention program against Salmonella infections in poultry
- Two consecutive proline substitutions in the fusion peptide of swine acute diarrhea syndrome coronavirus spike protein reduce cell-cell fusion
- Immune responses of the foot-and-mouth disease virus type Asia1 chimeric vaccine against Asia1 Shamir and Asia1/MOG/05 strains in pigs
- Combination of recombinant Brucella abortus dapB and gpm proteins induces host immunity against B. abortus 544 infection using a mouse model
- Effect of weakly acidic hypochlorous acid water on the treatment of murine dermatitis infected with Microsporum canis
참고문헌
교보eBook 첫 방문을 환영 합니다!
신규가입 혜택 지급이 완료 되었습니다.
바로 사용 가능한 교보e캐시 1,000원 (유효기간 7일)
지금 바로 교보eBook의 다양한 콘텐츠를 이용해 보세요!