Two consecutive proline substitutions in the fusion peptide of swine acute diarrhea syndrome coronavirus spike protein reduce cell-cell fusion

• 영문명: Two consecutive proline substitutions in the fusion peptide of swine acute diarrhea syndrome coronavirus spike protein reduce cell-cell fusion
• 발행기관: 한국예방수의학회
• 저자명: Bac Tran Le Jung-Eun Park
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.47, No.4, 185~189쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2023.12.31

영문 초록

Previous studies have shown that proline mutations in the heptad repeat region stabilize the coronavirus spike (S) protein in a pre-fusion state. To understand the impact of proline substitutions on the fusogenicity of the S protein, we engineered the swine acute diarrhea syndrome coronavirus (SADS-CoV) S protein with two proline substitutions (S-PP) and examined its fusogenicity using dual-split-protein based cell-cell fusion assay. Unlike the wild-type S (S-WT), S-PP rarely formed syncytia. Additionally, protein expression of S-PP was impaired compared to S-WT, as previously reported. Our results indicate that pre-fusion stabilized S protein is unable to induce membrane fusion and provide a better understanding of SADS-CoV S and vaccine antigen design.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

Fusogenicity Proline SADS-CoV Spike