형광영상을 이용한 옥수수 반수체 종자 선발 효율성 향상

• 영문명: Improvement of Selection Efficiency of Haploid Maize Seeds Using Fluorescence Imaging
• 발행기관: 한국육종학회
• 저자명: 김영욱 한정헌 김재영 김영태 김년희 이채원 이서연 김송림 김문종 류시환 이홍로 지현소 김경환 백정호
• 간행물 정보: 『한국육종학회지』Vol.54 No.4, 276~284쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.12.01

영문 초록

Many studies concerning breeding maize varieties are in progress in Korea and other countries. Double haploid technology is widely used for the development of commercial maize varieties worldwide, and has also been utilized in Korea since its introduction by the Maize Research Institute, Gangwondo. We performed a study to improve the efficiency of selecting haploid maize seeds using fluorescence imaging. It was verified that anthocyanin produced by the expression of R1-nj gene can be detected by fluorescence imaging, and we developed a high-throughput method for discriminating between haploid and diploid seeds. Compared with discriminating with naked eye, this method reduced the time for discriminating haploid and diploid maize by 91.7% and increased selection accuracy by 16.8% for haploid and 2.2% for diploid maize. This method enabled the acquisition of more haploid seeds and improved the efficiency of breeding research by shortening the time involved.

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키워드

haploid maize fluorescence image