아그로박테리움 매개 밀 형질전환

• 영문명: Agrobacterium-Mediated Transformation of Wheat
• 발행기관: 한국육종학회
• 저자명: 박은지 심재령 양유정 이샛별 김범기 김세원 이종열
• 간행물 정보: 『한국육종학회지』Vol.54 No.4, 358~368쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.12.01

영문 초록

Wheat (Triticum aestivum L.) is one of the world's three staple crops and accounts for approximately 20% of the total calories consumed by the world's population. It is known that wheat is a difficult crop to introduce foreign genes into, having a large genome (16 Gb) containing three highly related subgenomes (AABBDD). Owing to the low transformation efficiency of wheat, it is difficult to apply new technologies such as genome editing and basic research based on molecular biology, such as the discovery of useful genes and functional analysis. Recently, the completion of a wheat genome map by the International Wheat Genome Sequencing Consortium (IWGSC) and the development of a stable and reproducible wheat transformation system have accelerated research regarding the expression and control of useful genes. In this review, we introduce in detail the recently developed highly efficient Agrobacterium-mediated wheat transformation system and its applications in plant biotechnology, such as RNA interference (RNAi), overexpression, and gene editing using this system.

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agrobacterium tumefaciens genetic transformation wheat