히드록시우레아에 의한 멜라닌 생성에 미치는 세포 내 칼슘 및 산화질소의 역할

• 영문명: Involvement of Intracellular Ca²⁺ and Nitric Oxide in the Hydroxyurea-Induced Melanogenesis
• 발행기관: 대한약학회
• 저자명: 이용수(Yong Soo Lee)
• 간행물 정보: 『약학회지』제66권 제5호(2022년), 242~248쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2022.10.31

영문 초록

The molecular mechanism underlying hyperpigmentation as an adverse side effect of the anticancer drug, hydroxyurea (HU), was investigated in B16 melanoma cells. HU increased the melanin content and intracellular Ca²⁺ concentration in a dose-dependent manner. These effects were significantly reduced by treatment with the intracellular Ca²⁺ release blockers, dantrolene and 2-aminoethoxidiphenylborate (2-APB). Additionally, HU induced the production of nitric oxide (NO) in a time- and dose-dependent manner, and this was significantly blocked by inhibitors of the increase in intracellular Ca²⁺ levels(bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid/acetoxymethyl ester, dantrolene, and 2- APB). Furthermore, the HU-induced increases in NO and melanin production were significantly suppressed by NG-nitro- L-arginine methyl ester and 2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylinidazoline-1-oxyl-3-oxide, an NO synthase inhibitor and an NO scavenger, respectively. These results suggest that HU-induced melanogenesis through NO and an increase in intracellular Ca²⁺ levels may be involved in the mechanism of its hyperpigmentation effect.

목차

서 론(Introduction)

방 법(Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

감사의 말씀(Acknowledgment)

Conflict of Interest

References

키워드

B16 melanoma cell Hydroxyurea Hyperpigmentation Intracellular Ca²⁺ Melanogenesis Nitric oxide