LC-MS/MS를 이용한 건강기능식품 중 아스타잔틴 분석법 연구

• 영문명: Development and Validation of LC-MS/MS Method for Determination of Astaxanthin in Dietary Supplement
• 발행기관: 대한약학회
• 저자명: 박지은(Ji Eun Park) 오미현(Mi Hyune Oh)
• 간행물 정보: 『약학회지』제66권 제5호(2022년), 236~241쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2022.10.31

영문 초록

The Ministry of Food and Drug Safety is amending its test methods for dietary supplements (functional health food) to establish regulatory standards and specifications in Korea. In this regard, we continue to research analytical method development for the items to improve their sensitivity and selectivity. In this study, we have developed liquid chromatographic-tandem mass spectrometry for astaxanthin analysis. This method can be used to characterize and quantify dietary supplement formulations containing astaxanthin. The procedure was validated at 37.5-2,400 ng/mL. Standard calibration curves presented linearity with the coefficient of determination (r²)>0.999. The limits of detection and limits of quantitation were 16 and 49 ng/mL, respectively. The recovery results ranged between 91.9-97.4% at three different concentration levels with relative standard deviations (RSDs) of less than 3.6%. The precision results (RSDs) were obtained between 1.2-3.5%, and reproducibility (1.8%) was obtained. These are in accordance with the established validation criteria (Association of Official Analytical Chemists). Our research can provide scientific evidence to amend the astaxanthin test method for Health Functional Food Codes.

목차

서 론(Introduction)

방 법(Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

감사의 말씀(Acknowledgment)

Conflict of Interest

References

키워드

Astaxanthin LC-MS/MS Dietary supplement