RAW264.7 세포를 이용한 수은의 세포독성기전 및 수은에 대한 셀레늄의 방어기전에 대한 연구

• 영문명: A Study on Cellular Toxicity of Mercurials and Protection of Selenium against Mercurials in RAW264.7 Cell
• 발행기관: 한국환경보건학회
• 저자명: 고대하(Dai Ha Koh) 김준연(Jun Youn Kim) 염정호(Jung Ho Youm)
• 간행물 정보: 『1. 한국환경보건학회지』제25권 제1호, 117~124쪽, 전체 8쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1999.03.30

영문 초록

In present study, we elucidated the protective effects of selenium against the cytotoxic1ty of mercurial compounds(CH₃HgCl, HgCl₂) in RAW 264.7 cell and peritoneal macrophages from Balb/c mice. Mercurial compounds significantly decreased the cell viability and the synthesis of nitric oxide(NO) and cellular ATP in a dose-dependent manner. Selenium was not toxic at concentrations of 1-10 μM. But, selenium by itself was decreased the cell viability and the syntheses of NO and cellular ATP in dose-dependent manner above 20 μM conditions. However, in the selenium added conditions, the mercurial-induced cytotocixity was not observed. The protective effects of selenium against the cytotoxicity of mercurial compound depended on the concentration of added selenium to the culture conditions. We evaluated the mechanisms of protective effect of selenium against the mercury-induced cytotoxicity by the pretreatment experiments. In the selenium pretreatment, mercurial compounds still decreased the cell viability and the syntheses of NO and cellular ATP in a dose-dependent manner. These results indicated that the protective effect of selenium against the cytotoxicity of mercurial compounds was achieved by formation of some complex as such as SeHg or Se-Hg-protein. Though its mechanism was not clear, the protective role of selernium against the mercury toxicity would be exhibited in the immunological system.

목차

I. 서론

II. 실험재료 및 방법

III. 결과

IV. 고찰

키워드

CH₃HgCl HgCl₂ Selenium RAW264.7 Peritoneal Macrophage