광주지역 공공수역의 미생물 군집 다양성 및 항생제 내성에 관한 연구

• 영문명: A Study on Microbial Community Diversity and Antibiotic Resistance in Public Waters in Gwangju
• 발행기관: 한국환경보건학회
• 저자명: 김선정 박지영 김승호 임민화 유지용 한규성 박세일 서광엽 조광운
• 간행물 정보: 『한국환경보건학회지』제50권 제2호, 93~101쪽, 전체 9쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2024.04.30

국문 초록

Background: As pollutants caused by non-point sources flow into rivers, river water quality monitoring for fecal pollution is becoming increasingly important. Objectives: This study was conducted to investigate the distribution of microbial communities in the Yeongsangang River water system and sewage treatment plants in Gwangju and to evaluate their antibiotic resistance. Methods: In the experiment, samples were distributed to five selective media at each point and then cultured for 18 to 24 hours. When bacteria were observed, they were sub-cultured by size and shape and identified using MALDI-TOF MS equipment. When identification was completed, 17 types of antibiotic susceptibility tests were performed using VITEK II equipment, focusing on gram-negative dominant species among the identified strains. Results: During the study period, a total of 266 strains were isolated from 39 samples. Gram-positive bacteria were 37 strains in four genera, or 13.9% of the total, and Gram-negative bacteria were 229 strains in 23 genera, or 86.1% of the total. Antibiotic susceptibility testing of 23 strains, the major dominant species, showed that one strain (4.3%) was resistant to only one antibiotic, and two strains (8.7%) were 100% susceptible to the 17 antibiotics tested. The other 20 strains (87.0%) were multidrug resistant bacteria resistant to two or more antibiotics. There were various types of multidrug resistance. Among them, penicillin and cephalosporin series showed the highest resistance. Conclusions: Based on the results of this study, it was found that the bacterial community structure changed according to regional and environmental factors, and it was judged that continuous research such as genetic analysis of antibiotic-resistant bacteria present in natural rivers is necessary.

키워드

Non-point pollution source fecal pollution MALDI-TOF MS microbial community antibiotic resistance