3중 염색체 Probe의 FISH 기법으로 분석한 Bleomycin에 의한 사람 림프구의 염색체이상

• 영문명: Bleomycin Induced Chromosome Rearrangement Detected by Fluorescence in situ Hybridization Using Triple Combination of Composite Whole Chromosome-specific Probes in Human Lymphocyte
• 발행기관: 한국환경보건학회
• 저자명: 김수영(Su Young Kim) 정해원(Hai Won Chung)
• 간행물 정보: 『1. 한국환경보건학회지』제25권 제1호, 83~88쪽, 전체 6쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1999.03.30

영문 초록

Chromosome rearrangements induced by bleomycin were identified by fluorescence in situ hybridization (FISH) with triple combination of composite whole chromosome-specific probe for chromosome 1, 2 and 4. Triple combination of chromosome-specific probe covers larger proportion of DNA content in the whole genome, and higher validity in scoring chromosome rearragements can be achieved than the single chromosome-specific probe. Bleomycin treatment resulted in a dose dependent increase of all type of chromosome rearrangements scored by Protocol for Aberration Identification and Nomencluture Terminology (PAINT) System. Translocation was more frequently observed than dicentric chromosomes. And Bleomycin treatment has resulted in complex exchanges and cells with multiple aberrations.

목차

I. 서론

II. 재료 및 방법

III. 결과

IV. 고찰

V. 요약 및 결론

키워드

Chromosome rearrangements bleomycin triple combination probe FISH(fluorescence in situ hybridization)