독소원성대장균이 생산하는 장독소의 검색을 위한 RPLA 법과 PCR기법의 감도비교

• 영문명: Comparison of Sensitivity of Detection for Enterotoxigenic Escherichia coli Enterotoxin by use of the Reversed Passive Latex Agglutination and the Polymeras Chain Reaction
• 발행기관: 한국환경보건학회
• 저자명: 정희곤(Hee Kon Jung)
• 간행물 정보: 『1. 한국환경보건학회지』제25권 제1호, 6~9쪽, 전체 4쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1999.03.30

영문 초록

Detection for heat-labile enterotoxin(LT) of enterotoxigenic Escherichia coli(ETEC) by use of the reversed passive latex agglutination(RPLA) was positive reaction from 2-fold(50 ng/ml) to 64-fold dilutions in EC81 and EC83. The polymerase chain reaction(PCR) which was using LT gene-specific primers of ETEC with a detection limit equivalent from 1 ng/㎕ to 1 pg of a DNA fragment of 417-bp in EC81 and EC83. The PCR provided a rapid and more sensitivity tool for the detecting LT of ETEC compare to the RPLA.

목차

I. 서론

II. 실험재료 및 방법

III. 결과 및 고찰

IV. 결론

키워드

E. coli LT RPLA PCR