바이오에너지용 이질3배체 억새(Miscanthus × giganteus) 품종육성을 위한 물억새(M. sacchariflorus)와 참억새(M. sinensis) 출수기 단축과 단교배 방법

• 영문명: Shorten heading date of M. sacchariflorus and M. sinensis and single crossing method to breed Miscanthus × giganteus cultivar for bioenergy production
• 발행기관: 한국자원식물학회
• 저자명: 문윤호(Moon, Youn-Ho) 김광수(Kim, Kwang-Soo) 차영록(Cha, Young-Lok) 이지은(Lee, Ji-Eun) 권다은(Kwon, Da-Eun) 강용구(Kang, Yong-Ku)
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2018년도 추계학술대회, 51~51쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.10.31

영문 초록

Development of herbicide resistant transgenic legume plants through Agrobacterium-mediated transformation has been worked in many previous studied. Plant regeneration after infection is the important step to obtain successful transgenic plants. Many attempts try to find the optimum media condition for plant regeneration after infection. However, the transformation efficiency of legume plants is still low. In this study, regeneration of some Korean legume species including two soybean cultivars (Dawon and Pungsan) and pea have been done with organogenesis which is used various kind of explants such as cotyledonary-nodes in soybean and bud-containing tissue in pea. We developed the optimum media condition for plant regeneration regulators under Agrobacterium-mediated transformation using different kind and various concentration of plant growth. As the results, B5 medium containing 2 mg/L of 6-benzylaminopurine was selected in this study for the optimum plant regeneration media. The segments were inoculated with Agrobacterium suspension harbored an IG2 vector containing bar gene which confers resistance to phosphinotricin (PPT) in 3, 5 and 7 days. The transformation efficiency was achieved in Dawon 3.03 % and pea 1.46 % with co-cultivation period of 7 days which is showed a high number of GUS positive expression period.

키워드

Agrobacterium Pea Plant regeneration Plant transformation Soybean