Integrated Korean Flora Database: A versatile web-based database for dissecting flora investigations with climate data

• 영문명: Integrated Korean Flora Database: A versatile web-based database for dissecting flora investigations with climate data
• 발행기관: 한국자원식물학회
• 저자명: Yeon, Jihun Kim, Yongsung Kim, Hyejeong Kim, Juhyun Park, Jongsun
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2018년도 추계학술대회, 32~32쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.10.31

영문 초록

This study was conducted to examine the role of the transcription factors (TFs) (RsMYB1 and mPAP1+B-Peru) in the regulation of anthocyanin biosynthesis in the ornamental torenia cv. Kauai rose. In this study, we could produce several putative transgenic lines overexpressing the TFs via Agrobacterium-mediated transformation, and presence of the TFs in the randomly selected five transgenic lines was confirmed using polymerase chain reaction (PCR). According to results of reverse transcription-PCR analysis (RT-PCR), the expression of the TFs in all transgenic lines and of the anthocyanin structural genes (CHS, F3H, DFR, and ANS) in all transgenic lines and WT plants were distinctly detectable. However, transcript levels of the structural genes expressed in the transgenic lines overexpressing TFs were significantly higher than those expressed in WT plants. Therefore, it is suggested that anthocyanin content in flowers of the transgenic torenia would be significantly higher than that in flowers of WT plants. Moreover, these results indicate that the TFs (RsMYB1 and mPAP1+B-Peru) could be exploited as potential anthocyanin regulatory TFs to enhance anthocyanin content in the other horticultural plants.

키워드

Torenia RsMYB mPAP1+B-Peru Anthocyanin