학술논문
Comparison of Pedigree- and DNA-based Genetic Similarity among Korean Soybean Cultivars
이용수 4
- 영문명
- 발행기관
- 한국육종학회
- 저자명
- Seung Keun Jong Seong Hun Kim Sun Hee Woo Hong Sig Kim
- 간행물 정보
- 『한국육종학회지』Vol.38 No.1, 19~25쪽, 전체 7쪽
- 주제분류
- 농수해양 > 기타농수해양
- 파일형태
- 발행일자
- 2006.02.28
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국문 초록
영문 초록
Information on genetic diversity and relationships among the breeding materials is essential to the plant breeders in the efficient improvement of crops. The objective of this study was to determine the consistency between two methods of measuring genetic similarity (GS): the Pedigree-based genetic similarity (GSped) and the DNA-based genetic similarity (GSDNA), among 86 Korean soybean(Glycine max (L.) Merr.) cultivars bred between 1933 and 2002. GSped was estimated from coefficient of parentage (CP) among 86 Korea-bred soybean cultivars representing different eras, breeding methods and their utilization. Polymerase chain reaction (PCR) with twenty simple sequence repeat (SSR) markers was used for estimation of GSDNA for the same set of cultivars used for estimation of GSped. The mean of 3,655 GSped pairwise comparisons was 0.0314 with the range from 0.000 to 0.750, with higher distribution in the region of GS < 0.05. In contrast, GSDNA with the mean of 0.1716 was distributed more normally and mostly between 0.10 and 0.15. In spite of substantially different means and distributions of the two diversity measurements, a significant correlation (r = 0.256, P < 0.01) was detected between the two GS matrix. Also, cluster analyses based on average linkage algorithm for GSped and unweighed pair group method for GSDNA were made to compare the cultivar groups classified by the two methods of GS estimation. The ten groups classified by GSped included 2 to 22 cultivars, while the 7 groups classified by GSDNA included 2 to 25 cultivars. GSDNA may provide more accurate estimate of true genetic resemblance among soybean cultivars. But the significant correlation between two estimates indicated that GSped will continue to be useful to inexpensively identify diverse parents in a breeding program.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DICUSSION
LITERATURES CITED
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