Notch1 발현이 NCI-H460 폐암원형체의 침윤성 및 항암제 내성 발현에 미치는 기전 분석

• 영문명: Notch1 Expression Regulates the Invasiveness and Drug Resistance in Sphere-forming NCI-H460 Cancer Cells
• 발행기관: 대한약학회
• 저자명: 윤지은(Jieun Yun)
• 간행물 정보: 『약학회지』제62권 제6호(2018년), 381~387쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2018.12.31

영문 초록

Drug resistance is an obstacle to chemotherapy in non-small cell lung cancer caner (NSCLC). It has been reported cancer stem cells are regarded as the mediators of drug resistance, metastasis, and/or recurrence. Sphere-forming cells isolated from cancer cell lines are identified as cancer stem cells and broadly utilized for cancer stem cell studies. In this study, sphere-forming cells were isolated from NCI-H460 (H460) cells, the NSCLC cell line. The H460 spheres demonstrated increased invasion capacity as compared with H460 adherent cells and also appeared to be resistant to doxorubicin and cisplatin which are chemotherapy drugs. The mechanism involves in part through Notch signaling and Slug, known as a direct target of Notch1. The expression of Notch1 and Slug was remarkably higher in H460 spheres compared to H460 adherent cells. In addition, suppression of Notch1 expression, accompanied by decreased Slug expression, reversed the increased invasion capacity and the acquired drug resistance in H460 spheres. These findings suggest that Notch1 is a potential therapeutic target for cancer stem cells as a strategy for resolving metastasis and resistance to anticancer drugs.

목차

서 론(Introduction)

실험방법(Experimental Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

키워드

Cancer stem cells Notch signaling Slug Drug resistance Invasion