Animals, which were used in experimental field at home in 2017, amounted to about 310 animals. It has been 10 years since the Animal Protection Law took effect in our country. But diverse animal experiments are under way for an inevitable reason such as proving the safety of new drug and household items. Accordingly, it is being raised the insistence as saying of needing to prevent unnecessary animal sacrifice of being used in testing. Also, with being developed a method such as simulation experiment and cell testing available for substituting animal experiment according to the scientific development, the opinion is getting stronger as saying that the almighty animal-testing principle should be sublated. The animal testing cannot have expectation simply for an experimenter s ethicality. Even the regulation appropriate for this is needed. It is a fact that the regulation on animal experiment is very lacking in reality, and that the government and the political circles are passive in the institutional and policy-based improvement of animal testing for a reason of economy and corporate freedom. EU is being tightened the efforts to research into, develop and carry out the alternative animal-testing method that is safer and more scientific than animal experiment. In addition, it is being specified through the legislation based on EU s guidelines and through the decision case of ECJ(Court of Justice of the European Union). As the preferential activity for animal protection, EU is applying the guidelines of prohibiting animal experiment to the field of cosmetics. This study is aimed to consider the contents pertinent to the legal development of animal protection in EU and to the ban on animal testing in the field of cosmetics focusing on the guidelines enacted by the EU institution and on the decision case in ECJ.