방사면역확산효소시험(RIDEA)을 이용한 Japanese B encephalitis virus 항체검출에 관한 연구

• 영문명: Evaluation of radial immunodiffusion enzyme assay(RIDEA)for detection of antibody to Japanese B encephaltis virus
• 발행기관: 한국예방수의학회
• 저자명: 황영옥(Young Ok Hwang) 전무형(Moo Hyung Jun)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.17, No.3, 307~319쪽, 전체 13쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1993.12.30

영문 초록

In order to establish a rapid, sensitive and specific diagnostic method for detection of antibody to Japanese B encephalitis virus(JEV), a radial immunodiffusion enzyme assay(RIDEA) was designed and standardized. The diagnostic efficacy of the established RIDEA was compared with that of the standard hemagglutination inhibition(HI) test for JEV. The results obtained through the experiments were summarized as follows. 1. To determine the optimal concentration of JEV antigens in RIDEA, the antigen concentration from 0.5ug/ml to 5ug/ml were examined, and 2.5ug/ml was found to be the optimal antigen concentration. 2. It was found that the optimal time for antigen coating on the surface of polystyrene dish was 3 hours, and that the optimal time for antigen-antibody reaction were 6 hours for human serum and 15 hours for swine serum. 3. By the studies on effects of agar-gel substances, it was evident that the RIDEA values were slightly increased in agarose as compared with Noble agar. 4. When the effect of adsorption of test sera to various mouse tissue homogenates were studied, the specificity of RIDEA significantly increased in the serum adsorbed to the tissues of suckling mouse brain. 5. When the distribution of the values of standardized RIDEA was compared with that of the HI titers, the RIDEA values over 5.Omm corresponded to HI titer over 20, the values 4.0~4.9mm, to HI titer 10, and the values less than 4.0mm, to HI titer less than 10. The reproducibility of the established RIDEA was calculated to be 95.0%. 6. The relationship between the HI titer and the values of RIDEA was shown the high correlation coefficiency with human sera(r=0.93, p＜0.001, Y=0.03X+4.4) and with swine sera(r=0.84, p＜0.001, Y=0.01X+5.8).

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Radial immunodiffusion enzyme assay(RIDEA) Detection of Antibody Japanese B encephalitis Virus