Phanerochaete chrysosporium의 액체 배양 및 Lignin Peroxidase 생산

• 영문명: Submerged Culture of Phanerochaete chrysosporium and Lignin Peroxidase Production
• 발행기관: 강원대학교 산업기술연구소
• 저자명: 박세근(Park Se Keun) 정명선(Jeong Myoung Sun) 김영관(Kim Yeong Kwan)
• 간행물 정보: 『산업기술연구』vol.21, 347~353쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2001.09.30

영문 초록

This study characterizes the growth of white rot fungi Phanerochaete chrysosporium IFO 31249 and lignin peroxidase(LiP) activity in different submerged culture media. P. chrysosporium was grown in the form of pellet of various sizes from a spore inoculum under shaking liquid culture condition. While the growth of mycelia was higher under the nitrogen-sufficient culture than under the nitrogen-limited culture, ligninase activity was relatively lower. The lignin peroxidase appeared in nitrogen-limited culture and was suppressed by excess nitrogen. High level(40U/l) of lignin peroxidase activity was obtained in the growth medium containing 1.5mM veratryl alcohol, a secondary metabolite of P. chrysosporium. Lignin peroxidase production was not observed under conditions of nitrogen sufficiency or in balanced media, suggesting that control parameters could increase the activity by manipulating the secondary metabolism.

목차

Abstract

1. 서론

2. 재료 및 방법

3. 결과 및 고찰

4. 결론

참고문헌

키워드

액체 배양 lignin peroxidase 활성도 Phanerochaete chrysosporium submerged culture lignin peroxidase activity