LC-MS/MS를 이용한 우유 중 설파제의 동시 잔류 분석법

• 영문명: Multi-residue method for simultaneous determination of sulfonamide residues in milk by Liquid chromatography with tandem mass spectrometry
• 발행기관: 한국예방수의학회
• 저자명: 임채미(Chae Mi Lim) 권현정(Hyun Jeong Kwon) 조병훈(Byung Hoon Cho) 박수정(Su Jeong Park) 정갑수(Gap Su Chung) 손성완(Seong Wan Son)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.34, No.3, 245~254쪽, 전체 10쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 2010.09.30

영문 초록

A simple, selective and sensitive procedure for the confirmation of 14 sulfonamide antibacterials in milk was developed. The milk samples were homogenized, extracted and deproteinized by acetonitrile and defatted by n-hexane. Analysis was performed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) in positive mode for all 14 analytes. Mass spectral acquisition was performed in the multi reaction monitoring mode (MRM), selecting two structurally significant transitions per compound. The calibrations were performed in sample matrixes and the interference effect of sample matrixes on the ionization was effectively eliminated. Good linear relationship (R2=0.992~0.999) were observed at 6 concentrations of 2.5~100 ng/g. Satisfied recoveries (86.3~110.2%) of all sulfonamides were demonstrated in spiked milk at three levels from 5 to 20 ng/g. The limits of quantitation (LOQs) for sulfonamides ranged from 0.25~2.1 ng/g.

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Sulfonamides LC-MS/MS Multi-Residue Milk