돼지 흉막폐렴균의 유행 혈청형과 biofilm 분포도

• 영문명: Original Article : Distribution of serotype and biofilm of Actinobacillus pleuropneumoniae isolated from pigs
• 발행기관: 한국예방수의학회
• 저자명: 신동호(Dong Ho Shin) 변재원(Jae Won Byun) 김하영(Ha Young Kim) 김대근(Dae Keun Kim) 장원남(Won Nam Jang) 문운경(Oun Kyoung Moon) 이오수(O Soo Lee) 정병열(Byeong Yeal Jung)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.35, No.1, 7~12쪽, 전체 6쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 2011.03.30

영문 초록

The present study was conducted to investigate serotype distribution and biofilm formation of Actinobacillus (A.) pleuropneumoniae isolated from pneumonic lungs of pigs. A total of 37 A. pleuropneumoniae were isolated between January 2009 and June 2010. Serotypes of A. pleuropneumoniae isolates were determined using two different PCRs. The majority of isolates belonged to serotype 5 (n=31, 83.8%), and the others belonged to serotype 1 (n=4, 10.8%), and 2 (n=2, 5.4%), respectively. The ability of biofilm formation of the isolates was also determined by quantitative microtiter plate assay. Biofilm formation was observed in both 23 (62.2%) of the 37 field isolates and seven (43.8%) of the 16 reference strains. On the other hand, biofilm formation was various according to the serotypes: 20 (64.5%) of serotype 5, and three (75.0%) of serotype 1. However, two isolates of serotype 2 did not produce the biofilm in this study. Consequently, A. pleuropneumoniae serotype 5 was the most frequently detected (83.8% of the isolates), and 23 (62.2%) of 37 isolates exhibited biofilm positive phenotype in this study.

키워드

Actinobacillus pleuropneumoniae Biofilm PCR Pig Serotype