재조합 효모균의 회분배양 최적화의 생산성 향상

• 영문명: Enhanced Production of Peroxidase(PA1) and Culture Optimization of Recombinant Saccharomyces Cerevisiae
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 김태효(Tae Hyeo Kim)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제21권 2호, 57~63쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2016.06.30

영문 초록

The study on enhanced production of peroxidase(POD) and culture optimization of recombinant S. cerevisiae GPD-PODl was investigated with 5L jar fermentor. The peak of extracellular POD activity was detected at 20 h. The secrered yield of POD activity was found to be 87%. The maxirnal and stable POD activity was obtained with yeast nitrogen base w/o amino acid 6.7 g/L at 30℃, pH 8. The POD activity was increased up to 73-fold in a flask cultivation and enhanced by more than 155-fold in a laboratorical scale fermentor cultivation. The resultant maximum POD activity was obtained with respect to the control process of the respiratory quotient.(RQ) Additional improvement is likely to be obtained through a refinement of biotic control strategies.

목차

Abstract

Ⅰ. 서론

Ⅱ. 재료 및 방법

Ⅲ. 결과 및 고찰

Ⅳ. 결론

참고문헌

키워드

과산화효소 배양 최적화 재조합 효모 Peroxidase Culture Optimization Recombinant Yeast