자폐증 유사증상 발현 마우스의 점액 면역에 대한 연구

• 영문명: Evaluation of Mucosal Immunity in BTBR T+tf/J Mice Resembling Autism Spectrum Disorder
• 발행기관: 한국환경보건학회
• 저자명: 황소련(So Ryeon Hwang) 조지훈(Ji Hoon Jo) 신경민(Kyeong Min Shin) 장윤영(Yun Young Jang) 김지연(Ji Youn Kim) 여경욱(Kyeong Uk Yeo) 김형아(Hyoung Ah Kim) 허용(Yong Heo)
• 간행물 정보: 『한국환경보건학회지』제38권 제6호, 541~549쪽, 전체 9쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2012.12.30

영문 초록

Objectives: This study was undertaken in order to evaluate a potential mechanism involved in gastro-intestinal problems observed in autistic subjects and uses an animal model of autism investigation. Methods: BTBR T+tf/J, a mouse strain with typical socio-behavioral characteristics of autistic subjects and FVB mice with highly social behaviors as the control strain were used. Both genders of mice aged three weeks and six months were used from four separate litters for each strain. Serum was prepared following cardiac puncture, and mesenteric lymph nodes were collected for in vitro stimulation and enumeration of major immune cell proportion. Results: The level of serum IgA was significantly enhanced in six-month-old BTBR mice compared with threeweek- old BTBR, which was not observed with the FVB control mice. The serum IgE level was also higher among BTBR mice than among age-sex matched FVB mice, respectively. Considering the ratio of interleukin-4 vs interferon-gamma production from mesenteric lymph node T cells, skewedness toward type-2 reactivities was observed. In addition, the proportion of B cells in mesenteric lymph nodes was significantly higher in BTBR mice than in FVB mice. Conclusion: Upregulation of mucosal immunity related with enhanced type-2 immune reactivity observed in BTBR mice could be involved with the etiology of gastro-intestinal abnormalities in autism.

키워드

autism mucosal immunity BTBR T+tf/J mice IgA