LC-MS/MS를 이용한 메틸페니데이트 및 대사체 정량분석에서 검량선 작성을 위한 회귀모델, 직선성 및 가중계수 평가

• 영문명: Assessment of Regression Model, Linearity and Weighting Factor for Preparation of Calibration Curve in the Quantitative LC-MS/MS Measurements of Methylphenidate and its Metabolite
• 발행기관: 대한약학회
• 저자명: 김진영(Jin Young Kim)
• 간행물 정보: 『약학회지』제60권 제6호(2016년), 304~311쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2016.12.30

영문 초록

Preparation of calibration curve is one of the main sources of measurement uncertainty for quantitative determinations. A LC-MS/MS method for the quantification of urinary methylphenidate and retalinic acid has been evaluated by observing concentration-response curves, linearity and goodness of fit analysis. The calibration functions were calculated and fitted by linear and quadratic regression models with three different weighting factors. Also the correlation coefficient, the residuals plot and F-test were examined for the linearity of calibration curve. In this study, weighted (1/x²) linear regression was found to give more accurate results and was therefore selected.

목차

Abstract

서론

실험방법

실험결과 및 고찰

결론

References

키워드

quantification regression model linearity weighting factor LC-MS/MS