망막신경절세포에서 헴산화효소 발현에 의한 Betaxolol의 세포보호효과

• 영문명: Neuroprotective Effects of Betaxolol Mediated by Heme Oxygenase-1 Induction in RGC-5
• 발행기관: 대한안과학회
• 저자명: 차재봉(Jae Bong Cha) 권민영(Min Young Kwon) 정수월(Su Wol Chung) 우제문(Je Moon Woo)
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume57,number1, 113~119쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2016.01.30

영문 초록

Purpose: To evaluate the neuroprotective effects of betaxolol (betaxolol hydrochloride) under hypoxic conditions using retinal ganglion cells (RGC-5) and determine whether heme oxygenase-1 (HO-1) expression exerts cytoprotective effects. Methods: In this study, cultured RGC-5 cells were incubated with different concentrations of betaxolol hydrochloride (0.1 μM, 1μM or 5 μM) and with 10 μM zinc protoporphyrin (ZnPP), in a hypoxia incubator (1% O2, 5% CO2, 94% N2) for 48 hours and the cell viability of each group was determined. Additionally, cell viability was measured after RGC-5 cells were incubated with 5 μM of brinzolamide (Azopt？？), brimonidine tartrate (Alphagan？？) or travoprost (Travatan？？). RGC-5 cells were divided into three groups and incubated under three different conditions, normoxia group (20% O2, 5% CO2), hypoxia group (1% O2, 5% CO2) and the group with 5 μM of Betoptic S？？ treated under hypoxic conditions (hypoxia, Betoptic S？？). After incubation for 4, 8, 12 and 24 hours, HO-1 expression was analyzed using Western blotting. Results: Cell viability significantly increased in RGC-5 cells treated with Betoptic S？？ compared with other antiglaucoma agents. Increased levels of HO-1 expression indicate its relevance in cell viability. Furthermore, increased RGC-5 cell viability by Betoptic S？？ was significantly reduced in the HO-1 inhibitor ZnPP-treated group. Conclusions: We reaffirmed the known cytoprotective effects of Betoptic S？？ and the results suggests that HO-1 expression exerts cytoprotective effects against hypoxia.

키워드

Betaxolol Heme oxygenage-1 Hypoxia Retinal ganglion cells