각막상피제거술 후 흰쥐의 각막과 눈물샘에서의 신경성장인자의 변화

• 영문명: Expression of Nerve Growth Factor during Corneal Epithelial Wound Healing in Rats
• 발행기관: 대한안과학회
• 저자명: 신혜영 양지욱 이영춘 김수영,Hye Young Shin, MD, Ji Wook Yang, MD, Young Chun Lee, MD, PhD, Su Young Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume52,number5, 597~602쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.05.15

영문 초록

Purpose: To evaluate the expression of nerve growth factor (NGF) in the rat cornea and lacrimal gland before and after corneal epithelial wounding. Methods: Twenty-nine Sprague-Dawley male rats were used in the present study. Corneal trephination was performed using a 4.0-mm diameter trephine before scratch and at 24, 48, 72 hours after debridement. The lacrimal gland was excised before scratch and at 24 hours after epithelial debridement. NGF levels of the excised cornea and lacrimal gland were measured in rat corneas by enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry staining was performed on rat corneas and lacrimal glands. Results: The NGF/total protein ratio (NGF/tP) increased after wounding in the cornea and lacrimal gland. NGF levels in the cornea significantly increased in the wounded group until the 2nd day after wounding (p < 0.05). After NGF concentration peaked on the 1st day, there was a progressive decline after wounding. Additionally, the NGF concentration in the lacrimal gland of the wounded group was significantly higher than that of the control group at 24 hours after epithelial debridement (p = 0.001). Immunohistochemistry staining showed that NGF staining was stronger in rat corneas and lacrimal glands after epithelial debridement than before. Conclusions: Expression of NGF increased in rat corneas and lacrimal glands after corneal epithelial wounding, which suggests that NGF may play an important role in corneal wound healing. J Korean Ophthalmol Soc 2011;52(5):597-602

키워드

Cornea, Lacrimal gland, Nerve growth factor, Rat