섬유주세포에서 Erythropoietin이 일산화질소의 생성에 미치는 영향

• 영문명: Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells
• 발행기관: 대한안과학회
• 저자명: 이승희 김재우,Seung Hee Lee, MD, Jae Woo Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume52,number12, 1514~1518쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.12.15

영문 초록

Purpose: To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR. Results: EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accompanied with increased eNOS activity. Conclusions: EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity. J Korean Ophthalmol Soc 2011;52(12):1514-1518

키워드

Erythropoietin, Nitric oxide, Trabecular meshwork cells