콘택트렌즈 착용시 사용하는 인공누액이 인체각막상피세포에 미치는 영향

• 영문명: Effect of Artificial Tears Used in Contact Lens-wearing Eyes on Human Corneal Epithelial Cells in Vitro
• 발행기관: 대한안과학회
• 저자명: 김수진 김경호 이지은 이종수,Su Jin Kim, MD, Kyong Ho Kim, MD, Ji Eun Lee, MD, PhD, Jong Soo Lee, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume51,number4, 588~597쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.04.15

영문 초록

Purpose: To investigate the biologic effects of topical ocular artificial tears used in patients wearing contact lens on in vitro corneal epithelial cells. Methods: The efficacies of the topical artificial tears IrisⓇ, IrisplusⓇ, Eyemiru contact pureⓇ, and Eye2OⓇ were evaluated using the MTT and wound healing assays. Cell damage was determined using lactate dehydrogenase (LDH), and the solution ingredients were analyzed. Cellular morphologies were examined by inverted light microscopy and transmission electromicroscopy. Results: Metabolic activity of corneal epithelial cells, as determined by the MTT assay, decreased in the IrisⓇ eye drop group, but those of the other groups were similar to that of the control. The LDH titers increased up to one hour after IrisⓇ eye drop use, and the increased level was maintained for 24 hours. The other three artificial tears showed similar low LDH titers to that of the control. Cellular migration was not observed, although cellular damage to the corneal epithelial cells, such as chromatin margination and cytoplasmic organelle swelling, was prominent with IrisⓇ use. Conclusions: Among four brands of topical artificial tear drops used among patients wearing contact lens, IrisⓇ caused markedly more severe damage to cultured human corneal epithelial cells than did IrisplusⓇ, Eyemiru contact pureⓇ, or Eye2OⓇ. J Korean Ophthalmol Soc 2010;51(4):588-597

키워드

Artificial tear, Corneal epithelial cell toxicity, LDH, MTT