망막색소상피세포에서 포도당이 반응성산소종의 생성에 미치는 영향

• 영문명: Effect of Glucose on the Production of Reactive Oxygen Species in Retinal Pigment Epithelial Cells
• 발행기관: 대한안과학회
• 저자명: 이재형 김재우,Jae Hyung Lee, MD, Jae Woo Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume51,number2, 276~281쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.02.15

영문 초록

Purpose: To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal pigment epithelial (RPE) cells. Methods: ARPE-19 cells were exposed to low glucose (5 mM) and high glucose (HG, 25 mM) for three days. Additionally, 50 μM N-acetyl cysteine (NAC) and 100 μM L-arginine were co-exposed. Productions of nitric oxide (NO), ROS, and superoxide were determined by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively. Results: HG increased production of NO, ROS, and superoxide. HG-induced cells increased production of superoxide and ROS but were suppressed by NAC and L-arginine (substrate for NO production) as an antioxidant. Conclusions: HG increased ROS production in RPE cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in RPE cells. J Korean Ophthalmol Soc 2010;51(2):276-281

키워드

High glucose, Oxidative stress, Reactive oxygen species, Retinal pigment epithelial cells