이차원 전기영동법을 이용한 굴절교정레이저각막절제술 후 눈물 내 단백질의 비교 분석

• 영문명: Comparative Analysis of the Tear Protein Expression After Photorefractive Keratectomy Using Two-Dimensional Electrophoresis
• 발행기관: 대한안과학회
• 저자명: 채종근 박성표 최태훈,Jong Keun Chae, MD, Sung Pyo Park, MD, Tae Hoon Choi, MD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume50,number5, 762~768쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.05.15

영문 초록

Purpose: To investigate the change in the tear protein composition of patients who underwent refractive surgery. Methods: Tear samples were collected before photorefrative keratectomy (PRK), on the first, the second, and the third postoperative day, and then a month after the operation from 40 eyes of 20 patients. These tear samples were analyzed using two-dimensional gel electrophoresis (2-DE). Matrix-associated laser desorption/ionization time of flight (MALDI-TOF) was employed for the identification of expressed proteins. Control tear samples were collected from 40 eyes of 20 healthy volunteers who had no history of ocular surgery or pathology. Results: On the first postoperative day, lipocalin-1 precursor, lipocalin-1, and lysozyme were up-regulated. On the second postoperative day, serum albumin precursor and serum albumin were up-regulated. The tears collected on the third postoperative day and after 1 month had similar protein expression levels to the control group. Lipocalin 1 precursor and lysozyme were up-regulated and down-regulated after reftactive surgery, respectively. However, each protein had a different molecular weight and isopotential point. Conclusions: The tear protein composition changed uniquely in the early postoperative period, and proteins with different isopotential points were detected after PRK. We hypothesized that the healing process might influence the expression of the tear proteins.

키워드

Matrix-associated laser desorption/ionization time of flight (MALDI-TOF), Photorefractive keratectomy (PRK), Tear proteins, Two-dimensional electrophoresis (2-DE)