전환성장인자-베타 억제제(TGF-βinhibitor)의 각막열상 후 각막혼탁 억제 효과

• 영문명: The Inhibitory Effect of TGF-βInhibitor on the Corneal Opacity After Corneal Laceration
• 발행기관: 대한안과학회
• 저자명: 이재환 김이태 오중협,Jae Hwan Lee, MD, PhD, Ei Tae Kim, MD, Jung Hyub Oh, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume50,number3, 450~461쪽, 전체 12쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.03.15

영문 초록

Purpose: To evaluate the effects of TGF-β inhibitor on the wound healing process after corneal laceration, and its inhibitory effect on corneal scar formation. Methods: Forty Lewis rats were randomly divided into one control and three experimental groups (groups I, II, and III). After partial-thickness vertical linear corneal incision, a diluted solution with 10, 25, and 50 μg of TGF-β inhibitor was instilled into each eye of groups I, II, and III respectively. Corneal haze was measured by using slit-lamp biomicroscopic examination. Using histopathologic examination, we compared the number of stromal keratocytes and the arrangement of regenerated collagen fibers. We also performed immunohistochemistry to confirm the differential expression of fibronectin and α-smooth muscle actin in each group. Results: Group III showed less corneal haze and more regular arrangement of regenerated collagen fibers than the other groups. The number of stromal keratocytes and immunoreactivity to fibronectin and α-smooth muscle actin decreased as the dose of TGF-β inhibitor increased. Conclusions: TGF-β inhibitor effectively reduced corneal haze during corneal healing processes after corneal laceration.

키워드

Corneal laceration, Corneal opacity, TGF-β inhibitor, Transforming growth factor-beta