각막기질세포에 대한 아칸트아메바각막염 치료제의 영향

• 영문명: Effect of Amoebicidal Agents on the Human Corneal Keratocytes in Vitro
• 발행기관: 대한안과학회
• 저자명: 이지은 이종수 최희영 유학선,Ji Eun Lee, M.D., Jong Soo Lee, M.D., Hee Young Choi, M.D., Hak Sun Yoo, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume48,number1, 125~134쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.01.31

영문 초록

Purpose: To evaluate the effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba cysts and cultured human keratocytes. Methods: Each well of two-fold diluted PHMB and chlorhexidine were treated on the Acanthamoeba cyst suspension of 5×104 cysts/ml for 8, 24, and 48 hours to measure the minimal cysticidal concentration (MCC) of each disinfectant and was exposed to the human corneal keratocytes of 5×104 cells/ml for same hours to measure the survival rate of keratocytes. Inverted phase-contrast micrograph and electron microscopy for observing the morphologic changes were evaluated. Results: MCC of PHMB was 9.42 ?g/ml, 5.62 ?g/ml, and 2.37 ?g/ml, and chlorhexidine was 24.32 ?g/ml, 10.02 ?g/ml, and 7.02 ?g/ml respecitvely in 8, 24, and 48 hours. The survival rate of keratocytes at MCC was 91.7%, 64.6%, and 49.7% in PHMB of which significant decreases were found at 24 and 48 hours, and 95.7%, 90.6%, and 78.1% in chlorhexidine of which significant decrease was only found at 48 hours. The higher the concentration of disinfectants, cysts and keratocytes demonstrated more damaged appearance. Conclusions: The amoebicidal efficacy of PHMB and chlorhexidine was similar. However, in consideration of toxic effect on keratocytes by disinfectants, chlorhexidine is suggested to be more clinically useful than PHMB.

키워드

Acanthamoeba, Chlorhexidine, Human corneal keratocyte, Minimal cysticidal concentraion, Polyhexamethylene biguanide