사람 망막아세포종 세포에 발현된 니코틴성 수용체의 특성

• 영문명: Characteristics of Nicotinic Receptor Expressed in Human Retinoblastoma
• 발행기관: 대한안과학회
• 저자명: 김대란 차승규 공인덕 정세환 라상훈,Dae-Ran Kim, Ph.D., Seung-Kyu Cha, Ph.D., In-Deok Kong, M.D., Se-Hwan Jung, M.D. Sang Hoon Rah, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume46,number6, 1060~1067쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2005.06.30

영문 초록

Purpose: To identify the characteristics and physiological function of the nicotinic receptor expressed in human retinoblastoma cells. Methods: We measured possible nicotinic signaling in WERI-Rb-1 cells using the Ca2+ imaging technique and the patch clamp method. Results: 1) Nicotine-induced [Ca2+]i rise arose entirely through Ca2+ influx, which was completely abolished by hexamethonium (100 μM). 2) Nicotine also induced remarkable depolarization from -56.6±3.7 mV to -29.6±3.6 mV (n=4) under current clamp mode, but it failed to directly activate the T-type Ca2+ channel expressed in retinoblastoma cells. Conclusions: Nicotinic activation can increase the intracellular calcium level through calcium influx in the undifferentiated retinoblastoma cells, which may play important roles in cell proliferation, differentiation, and cell death.

키워드

Calcium, Nicotinic receptor, Retinoblastoma cell