콜라겐 젤과 성장인자 전처치 및 피복재료가 다공성안와삽입물의 섬유혈관증식에 미치는 효과

• 영문명: Effect of Collagen Gel with Growth Factor and Wrapping Materials on Fibrovascularization of Porous Orbital Implant
• 발행기관: 대한안과학회
• 저자명: 이준영 김석영 유창국 이무석 이상렬 오중협 김성주,Joon-Young Lee, M.D., Suk-Young Kim, Ph.D., Chang-Kook You, Moo-Seok Lee, Sang-Yeul Lee, M.D., Ph.D., Jung-Hyub Oh M.D., Ph.D., Sung-Joo Kim M.D., Ph.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume46,number6, 1037~1051쪽, 전체 15쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2005.06.30

영문 초록

Purpose: To determine the effect of bFGF complexed collagen gel, which allows constant release of bFGF along with biodegradation of the collagen gel. The specific study purpose was to determine whether it can accelerate the fibrovascular ingrowth into wrapped HA-coated porous alumina and to verify the safety of new wrapping materials. Synthetic polyester-urethane (Neuropatch?) and lyophilized bovine pericardium (Lyoplant?) were compared to donor sclera for the fibrovascular ingrowth into HA-coated porous alumina. Methods: The experimental and control groups, each consisting of 9 rabbits were wrapped with each wrapping materials (3 rabbits per wrapping material). The experimental group underwent pretreatment of bFGF-collagen gel while the control group did not. The fibrovascular ingrowth was compared at 2 and 4 weeks after implantation. Western blot analysis was conducted at 4 weeks using antibodies against CD141 and laminin. The rate of fibrovascular ingrowth was fastest in orbital implant wrapped with Lyoplant?. Results: Histopathologic examinations at 2 weeks showed no differences in distance and percentage area of fibrovascular ingrowth. Histopathologic examinations at 4 weeks showed that pretreatment of bFGF-collagen gel increased the fibrovascular ingrowth in the experimental group. Western blot analysis on experimental group also showed that the expressions of CD141 and laminin were increased by bFGF-collagen gel, thereby indicating that the fibrovascular proliferations were accelerated by bFGF released from the complex. Conclusions: bFGF-collagen gel increased the rate and degree of fibrovascular growth into hydroxyapatite- coated porous alumina by releasing bFGF as the collagen gel biodegraded. Both Lyoplant? and Neuropatch? were evaluated as safe for substitution of the donor sclera.

키워드

Basic fibroblast growth factor (bFGF), Collagen gel, Hydroxyapatite-coated porous alumina, Wrapping materials