대장암 세포에서 5-FU(Fluorouracil)의 세포독성과 관련된 단백체 분석

• 영문명: Proteomic Approach to the Cytotoxicity of 5-FU(Fluorouracil) in Colon Cancer Cells
• 발행기관: 대한약학회
• 저자명: 이서영(Seo Young Lee) 송진수(Jin-Su Song) 노시훈(Si Hun Roh) 김근태(Geun Tae Kim) 홍순선(Soon-Sun Hong) 김희준(Hie-Joon Kim) 권성원(Sung Won Kwon) 박정일(Jeong Hill Park)
• 간행물 정보: 『약학회지』제53권 제3호 (2009년), 145~150쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.06.30

영문 초록

We evaluated cytotoxic effect based on the MTT assay and identified altered proteins in 5-FU(fluorouracil) treated HT29 cells using two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS. As proteins inducing apoptosis, siah binding protein 1 and p47 protein isoform a were up-regulated and tumor protein translationally-controlled 1 was downregulated by 5-FU treatment. And mannose 6 phosphate receptor binding protein 1 controls DNA mismatch repair system was increased. We suggest 5-FU promotes a cytotoxicity under the action of these proteins in colon cancer cells.

키워드

5-FU colon cancer proteomics MALDI-TOF/TOF