유전자재조합 인터페론 알파의 HPLC-PDA에 의한 함량분석

• 영문명: The Quantitative HPLC-PDA Method for Recombinant Interferon Alpha Analysis
• 발행기관: 대한약학회
• 저자명: 김지현(Gihyun Kim) 박지은(Jieun Park) 백승권(Seungkwan Paek) 김춘미(Choonmi Kim) 홍성화(Seunghwa Hong) 이화정(Hwajoung Lee) 손여원(Yeowon Sohn)
• 간행물 정보: 『약학회지』제53권 제3호 (2009년), 101~106쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.06.30

영문 초록

Recombinant interferon alpha-2a is an active formula for the treatment of various cancer cells like malignant melanoma and a variety of virus infection diseases like acute or chronic hepatitis. The bioassay system based on the measurement of virus inhibitory activity by interferon has been used for interferon analysis with low repeatability. Here, we developed the HPLC assay to measure reproducibly interferon alpha-2a protein content which is replaceable to the reported bioassay. This method separated interferon alpha-2a from its oxidized forms and human serum albumin used as excipients. The regression coefficient using interferon alpha-2a EP CRS is more than 0.9 in the range from 5 μg/ml to 200 μg/ml. The recovery result in the range from 15 μg/ml to 60 μg/ml is 97~104% and the precision is 0.2~1.7%. The interferon alpha contents of 5 products are about 30 μg/ml.

키워드

interferon alpha HPLC human serum albumin content