Diethylstilbestrol의 단핵구의 세포간 유착과정 조절효과

• 영문명: Modulatory Effect of Diethylstilbestrol on CD29-Mediated Cell-cell Adhesion in Monocytic U937 cells
• 발행기관: 대한약학회
• 저자명: 김병훈(Byung Hun Kim) 조재열(Jae Youl Cho)
• 간행물 정보: 『약학회지』제52권 제2호 (2008년), 111~116쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.04.30

영문 초록

Diethylstilbestrol (DESB) is a synthetic estrogen not only that routinely prescribed, but also that known to be a teratogen. In this study, we found a novel pharmacological feature that DESB is able to positively modulate CD29 (β1-integrin) function. Thus, DESB up-regulated homotypic cell-cell adhesion of monocytic U937 cell mediated by CD29. However, DESB did not increase the surface level of CD29 and its binding activity to ligand (fibronectin), according to flow cytometric analysis and cell-fibronectin adhesion assay. Instead, the DESB-mediated up-regulation of cell-cell adhesion was blocked by several signaling enzyme inhibitors. Treatment of U0126 [an extracellular signal-regulated kinase (ERK) inhibitor], SB20358 (a p38 inhibitor) or Rp-8-pCPT-cGMP (a protein kinase G inhibitor) clearly inhibited DESB-mediated up-regulation of cell-cell adhesion induced by DC29. However, estrogen receptor antagonist ICI 182,780 failed to abrogate DESB effect. Therefore, out data suggest that DESB may up-regulate CD29-mediated cell-cell adhesion via modulating intracellular signaling enzymes such as ERK, PKG, and p38, independent of estrogen receptor function.

키워드

diethylstilbestrol(DESB) cell-cell adhesion CD29 adhesion molecules