Electroblotting을 이용한 단일클론항체의 NH2-말단 아미노산 배열분석

• 영문명: NH2-terminal Amino acid Sequence Analysis of Monoclonal Antibody by Electroblotting Method
• 발행기관: 대한약학회
• 저자명: 남경수(Kyung Soo Nam) 장현욱(Hyeun Wook Chang) 정규찬(Kyu Charn Chung)
• 간행물 정보: 『약학회지』제34권 제1호 (1990년), 11~14쪽, 전체 4쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1990.02.28

영문 초록

NH2-terminal amino acid sequence analysis of monoclonal antibody is very important to identify gene family and diversities of antigen-antibody recognition. When we used the PVDF (Polyviriylidene difluoride) membrane blotting method, we could easily analyze NH2-terminal sequence of monoclonal-antibody which specifically binds to phosphatidylinositol 4,5-biphosphate. PVDF membrane is an ideal solid-phase support for sequence analysis, especially when used with electroblotting method. This method is superior to continual method and will be applied to the sequence analysis of picomole quantities of proteins by gel electrophoresis.

키워드

Monoclonal antibody PVDF membrane Electroblotting