인형 T세포 백혈병에 대한 단세포군 항체 생산에 관한 연구

• 영문명: Studies on Production of Monoclonal Antibodies Reactive with T-Cell Leukemia
• 발행기관: 대한약학회
• 저자명: 서병석(Byung Seok Seo) 김원배(Won Bae Kim) 최응칠(Eung Chil Choi) 김병각(Byong Kak Kim)
• 간행물 정보: 『약학회지』제31권 제5호 (1987년), 253~265쪽, 전체 13쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1987.10.30

영문 초록

To develop hybridomas secreting monoclonal antibodies to be used as unlimited sources of reagents indispensable for the diagnosis and treatment of leukemic malignancy, a monoclonal antibody was generated to human pre-T leukemia cells (Jurkat). Hybridomas were produced against Jurkat cell line by fusing spleen cells from hyperimmunized mice with murine plasmacytoma cells (P3 X 63Ag8.V653). One monoclonal antibody derived from this fusion, designated DMJ-2 was reactive with T-cell lines (Jurkat, Molt-4 and RPMI-8402) and normal peripheral E-rosette forming T cells, but unreactive with B-cell lines (Daudi, Nalm-6) and non-T, non-B cell line (K562). Conclusively DMJ-2 was reactive with mature and immature T-lineage lymphoid cells.