Farnesol의 HL-60 세포에 대한 세포독성과 활성산소 및 황산화효소 활성 변화

• 영문명: Reactive Oxygen Species and Antioxidant Enzyme Activities in Accordance
• 발행기관: 대한약학회
• 저자명: 임소윤(So Yoon Lim) 박시원(Sie Won Park)
• 간행물 정보: 『약학회지』제50권 제6호 (2006년), 372~380쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.12.31

영문 초록

Farnesol in fruits, vegetables, herbs and leaves acts as bioactive component with pervention of cancer and phychologica malaise. We investigated the cytotoxic effects of farnesol on human leukemic cell, HL-60 cells, by MTT assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Farnesol (0.1~50 μg/ml) exhibited cytotoxicities against HL-60 cells, the generation of reactive oxygen species such as O2 and H2O2 were found to be considerably increased. The most prominent augmentations of O2 and H2O2, superoide dismutase (SOD), glutathione peroxidase (GPx) and catalase activities of HL-60 cells treated with farnesol were measured. SOD and GPx activities were found to be remarkably elevated by addition of farnesol showing the best results of 273% and 167% of controls, respectively. All data suggest that farnesol may have played as an apoptosis inducer in HL-60 cells via production of reactive oxygen species (ROS) and HL-60 cells may have failed to overcome the damage of ROS on account of still deficient ROS scavengers including SOD and GPx.

키워드

famesol cytotoxicities HL-60 cells ROS antioxidant enzymes